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Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
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Published on: July 30, 2020

The physiological stimulus for the BarA sensor kinase.

Ricardo Gonzalez Chavez1, Adrian F Alvarez, Tony Romeo

  • 1Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.

Journal of Bacteriology
|February 2, 2010
PubMed
Summary
This summary is machine-generated.

The BarA/UvrY two-component system (TCS) links cell metabolism to gene regulation. Formate and acetate signal this TCS, controlling the Csr system

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Systems Biology

Background:

  • The BarA/UvrY two-component system (TCS) regulates gene expression via noncoding RNAs.
  • The Csr system, involving CsrA protein and CsrB/CsrC RNAs, controls posttranscriptional gene regulation.
  • The Csr system's link to cellular metabolic state was previously unclear.

Purpose of the Study:

  • To investigate the physiological stimuli that activate the BarA/UvrY TCS.
  • To determine how metabolic end products influence the Csr regulatory system.
  • To establish a connection between cellular metabolism and posttranscriptional regulation.

Main Methods:

  • Investigated the role of formate and acetate as signaling molecules.
  • Analyzed the activation of the BarA/UvrY TCS by metabolic end products.
  • Assessed the impact of these signals on CsrB and CsrC noncoding RNA transcription.
  • Examined the interaction between the Csr system and metabolic state.

Main Results:

  • Formate and acetate were identified as physiological stimuli for the BarA/UvrY TCS.
  • Activation of the BarA/UvrY TCS by these metabolites leads to increased CsrB and CsrC RNA levels.
  • The Csr system's activity is directly modulated by the cellular metabolic state through this signaling pathway.

Conclusions:

  • Metabolic end products formate and acetate act as signals for the BarA/UvrY TCS.
  • This signaling links cellular metabolic status to posttranscriptional regulation mediated by the Csr system.
  • The study elucidates a key mechanism connecting primary metabolism to global gene regulation.