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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Bacillus oceanisediminis sp. nov., isolated from marine sediment
Jianli Zhang1, Jiewei Wang1, Caiyuan Fang1
1School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Abstract:
A Gram-stain-positive, spore-forming, rod-shaped and aerobic bacterium was isolated from a sediment sample from the South Sea in China. The isolate, designated H2(T), grew at 4-45 °C (optimum 37 °C) and pH 6-10 (optimum pH 7.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major isoprenoid quinone was MK-7 and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown aminophospholipid. The major fatty acid was iso-C(15 : 0). The genomic DNA G+C content of strain H2(T) was 44.8mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a monophyletic clade with Bacillus firmus IAM 12464(T). DNA-DNA relatedness between the isolate and B. firmus ATCC 14575(T) was low (27.5 %). Strain H2(T) also had a phenotypic profile that readily distinguished it from its closest phylogenetic neighbours. It is evident from the combination of genotypic and phenotypic data that the organism should be classified in a novel species of the genus Bacillus, for which the name Bacillus oceanisediminis sp. nov. is proposed. The type strain is H2(T) (=CGMCC 1.10115(T) =JCM 16506(T)).
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