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Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Published on: April 24, 2018
Effects of alachlor on biochemical parameters of the freshwater fish, Channa punctatus (Bloch)
K S Tilak1, P Wilson Raju, M S Butchiram
1Department of Zoology, Acharya Nagarjuna University, Nagarjunanagar, India. proftilak@yahoo.com
Abstract:
The fresh water fish Channa punctatus (Bloch) were exposed to lethal and sublethal concentrations of a chloroacetanilide herbicide Alachlor and its commercial formulation Lasso 50% Emulsifiable Concentrate EC to study the impacts on some biochemical parameters - the energy dependent sources: such as glycogen, total proteins and metabolic enzymes: Aspartate amino transferase (AAT), Alanine amino transferase (ALAT), Lactate Dehydrogenase (LDH), Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA). The glycogen, total proteins, DNA, RNA were all decreased but the activity of the enzymes AAT, ALAT and LDH were all increased which is due to the toxic stress. The percentage decrease being more pronounced at lethal concentrations than at sublethal concentrations.

