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Updated: Jun 16, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors.

Cheng Sun¹, Kai-Hung Su, Jason Valentine

¹Nanoscale Science and Engineering Center, University of California, Berkeley, CA 94720, USA. c-sun@northwestern.edu

|February 4, 2010

Summary

This summary is machine-generated.

This study introduces a novel nanoplasmonic resonator (NPR) for highly sensitive protease activity measurement. This method achieves picomolar sensitivity, outperforming conventional assays for applications like prostate-specific antigen (PSA) detection.

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Area of Science:

Biochemistry
Nanotechnology
Analytical Chemistry

Background:

Protease activity assays are crucial for drug screening, diagnostics, and molecular profiling.
Conventional immunopeptidemetric assays (IMPA) suffer from low signal-to-noise ratios, limiting sensitivity at clinically relevant picomolar to nanomolar concentrations.

Purpose of the Study:

To develop a highly sensitive method for measuring protease activity using nanoplasmonic resonators (NPRs).
To enable real-time detection of proteolytically active prostate-specific antigen (paPSA) at low concentrations.

Main Methods:

Utilized nanoplasmonic resonators (NPRs) to significantly enhance Raman signals (6.1 x 10^10 times).
Developed a method for fast, real-time detection of protease activity.

Main Results:

Achieved a sensitivity level of 6 pM (0.2 ng/mL) for paPSA detection.
Demonstrated a wide dynamic range of 3 orders of magnitude.
Successfully detected paPSA in complex biological samples like extracellular fluid (ECF) with high specificity.

Conclusions:

Nanoplasmonic resonators offer a fast, sensitive, and accurate approach for protease activity detection.
This one-step method is suitable for analyzing very small sample volumes.
The technology holds promise for improved diagnostics and molecular profiling.