Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysregulated Proteins in Plasma Distinguishing Loeys-Dietz Syndrome from Other Heritable Thoracic Aortic Disease - An Explorative Study.

Scandinavian cardiovascular journal : SCJ·2026
Same author

Evolution of the drug sensitivity landscape of chronic lymphocytic leukaemia.

British journal of haematology·2026
Same author

Fast scanning in chest CT: Investigating clinical and technical image quality features.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2026
Same author

Von Willebrand disease.

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·2026
Same author

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·2026
Same author

Cryoglobulinemia Type II: Sustained Remission After B-Cell-Directed Therapy.

The American journal of case reports·2026

Related Experiment Video

Updated: Jun 16, 2026

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling
10:29

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling

Published on: April 3, 2026

Striking decrease in the total precursor B-cell compartment during early childhood as evidenced by flow cytometry and

Kristin Jensen1, Lana Schaffer, Ole K Olstad

  • 1Department of Pediatrics, Faculty Division Ullevål, University of Oslo, Oslo, Norway. kristin.jensen@medisin.uio.no

Pediatric Hematology and Oncology
|February 4, 2010
PubMed
Summary

B-cell development in bone marrow shows a transient increase in precursor B-cells during infancy, followed by a gradual decline into adulthood. This bone marrow kinetics differs from circulating B-cell levels in peripheral blood.

More Related Videos

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
14:06

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

Related Experiment Videos

Last Updated: Jun 16, 2026

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling
10:29

Mapping Infant Immunity with Minimal Input: Integrative Single-Cell and Multiomic Profiling

Published on: April 3, 2026

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
14:06

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

Area of Science:

  • Immunology
  • Developmental Biology
  • Hematopoiesis

Background:

  • Circulating B-cell counts plateau in infancy and decline gradually, but the underlying bone marrow precursor B-cell kinetics in humans remain poorly understood.
  • Understanding precursor B-cell pool dynamics is crucial for interpreting peripheral B-cell levels and immune development.

Purpose of the Study:

  • To investigate age-related changes in the human bone marrow precursor B-cell compartment.
  • To identify genes associated with precursor B-cell development and correlate them with recombination activating gene 1 (RAG1) expression.

Main Methods:

  • Analysis of bone marrow samples from healthy children and adults using flow cytometry to assess precursor B-cell populations.
  • Global gene expression profiling (Affymetrix) of bone marrow to identify age-related transcriptional changes, focusing on RAG1.
  • Bioinformatic analysis to find gene transcripts correlated with the RAG1 expression profile.

Main Results:

  • A significant, transient increase in total precursor B-cells was observed between 6-20 months of age, followed by a rapid decrease within the first two years.
  • Gene expression analysis revealed 54 genes highly correlated with RAG1, including 15 known B-lineage genes and 39 novel associated transcripts.
  • The transient increase in bone marrow precursor B-cells and RAG1 activity was not mirrored by a similar peak in peripheral blood B-cells.

Conclusions:

  • Human bone marrow precursor B-cell numbers exhibit distinct age-related kinetics, with a transient peak in infancy not reflected in peripheral blood.
  • The study identified novel genes associated with B-lineage development, providing new targets for understanding B-cell differentiation.
  • These findings enhance our understanding of B-cell homeostasis and immune maturation throughout childhood and into adulthood.