Related Experiment Video
Updated: Jun 16, 2026
Multiplex Cyclic Fluorescent Immunohistochemistry
Published on: January 26, 2024
Diagnosis of metastatic neoplasms: an immunohistochemical approach
1Department of Pathology, Mayo Clinic Florida, 4500 San Pablo Road, Jacksonville, FL 32224, USA. krishna.murli@mayo.edu
Context:
It is important to determine the type and/or site of origin of metastatic tumors for optimal clinical management.
Objective:
To summarize the use of currently available immunohistochemical markers in the evaluation of metastatic tumors.
Data Sources:
Review of relevant literature on immunohistochemical evaluation of tumors and the author's personal experience.
Conclusions:
Immunohistochemistry is an important ancillary technique for evaluation of metastatic tumors and should be used in the context of routine morphology and clinical information. While a single marker may be used to support a known or suspected site of origin, a carefully constructed panel is strongly recommended, particularly for tumors of morphologically uncertain lineage or origin.
