Related Experiment Video

Updated: Jun 16, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

Tn5090-like class 1 integron carrying bla(VIM-2) in a Pseudomonas putida strain from Portugal

C Santos1, T Caetano, S Ferreira

  • 1CESAM and Department of Biology, University of Aveiro, Campus de Santiago, Aveiro, Portugal.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|February 4, 2010
PubMed

Insights

Three Pseudomonas putida strains carrying the bla(VIM-2) gene were found in a hospital

Area of Science:

  • Microbiology
  • Environmental Science
  • Infectious Diseases

Background:

  • The bla(VIM-2) gene confers resistance to carbapenem antibiotics.
  • Hospital environments can harbor antibiotic-resistant bacteria.
  • Pseudomonas putida is an opportunistic pathogen found in various environments.

Purpose of the Study:

  • To investigate the presence and genetic context of bla(VIM-2) in Pseudomonas putida strains from a hospital sanitary facility.
  • To characterize novel integron structures associated with bla(VIM-2) in these isolates.

Main Methods:

  • Isolation and identification of Pseudomonas putida strains.
  • Molecular characterization of integrons and associated genes using PCR and sequencing.
  • Analysis of genetic arrangements, including transposons and insertion sequences.

Main Results:

  • Three Pseudomonas putida strains harboring bla(VIM-2) were isolated from a hospital sanitary surface.
  • Novel class 1 integrons were identified in two strains, one with a unique gene cassette array disrupted by IS1382.
  • A third strain exhibited a class 1 integron linked to a Tn5090-like transposon, a novel finding in Portugal.

Conclusions:

  • The study demonstrates the presence of bla(VIM-2} carrying Pseudomonas putida in a hospital environmental setting.
  • The findings highlight the role of integrons and transposons in the dissemination of carbapenem resistance genes.
  • Environmental surveillance is crucial for understanding the spread of antibiotic resistance in healthcare facilities.

More Related Videos

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System05:06

Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

Overview
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...