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Related Concept Videos

Aquaporins01:25

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
Reabsorption and Secretion in the DCT and Collecting Duct01:26

Reabsorption and Secretion in the DCT and Collecting Duct

The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal part of the DCT, along with the...
Salivary Glands and Saliva01:23

Salivary Glands and Saliva

The salivary glands, of which there are three pairs known as the parotid, submandibular, and sublingual glands, play a crucial role in maintaining oral health and initiating the digestive process. Positioned near the ears, beneath the masseter muscle, the parotid glands secrete saliva into the oral cavity through the parotid duct of Stensen. Meanwhile, the submandibular glands, located on the floor of the mouth, secrete saliva through channels named submandibular ducts. The sublingual glands,...

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Related Experiment Video

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Culturing Primary Rat Inner Medullary Collecting Duct Cells
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Published on: June 21, 2013

Aquaporin expression patterns in the developing mouse salivary gland.

Helga S Larsen1, Ann-Kristin Ruus, Hilde Kanli Galtung

  • 1Department of Oral Biology, University of Oslo, Oslo, Norway.

European Journal of Oral Sciences
|February 4, 2010
PubMed
Summary

This study reveals how aquaporins (AQPs), water channels, change in mouse salivary glands during development. Their varying presence suggests important roles in gland formation and function.

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Area of Science:

  • Physiology
  • Developmental Biology
  • Molecular Biology

Background:

  • Aquaporins (AQPs) are water channels crucial for physiological functions.
  • Their role in salivary gland development, particularly the mouse submandibular salivary gland (SMG), is not well understood.
  • Understanding AQP expression during SMG development is key to comprehending gland maturation.

Purpose of the Study:

  • To investigate the expression patterns of specific aquaporins (AQPs 1, 3, 4, 5, and 8) during prenatal and postnatal development of the mouse submandibular salivary gland (SMG).
  • To correlate AQP expression trends with developmental stages and potential physiological roles in SMG development.

Main Methods:

  • Utilized mouse SMG models at embryonic, early postnatal, and young adult stages.
  • Detected and quantified AQP transcripts using reverse transcription-polymerase chain reaction (RT-PCR).
  • Detected and quantified AQP proteins using western blotting.

Main Results:

  • Transcripts for AQPs 1, 3, 4, 5, and 8 were detected.
  • Proteins for AQPs 1, 3, 4, and 5 were detected, but AQP 8 protein was not.
  • Distinct transcript and protein expression patterns were observed for the various AQPs across developmental stages.
  • Expression trends varied significantly, suggesting stage-specific importance.

Conclusions:

  • Aquaporin presentation and expression patterns are dynamic during mouse SMG development.
  • These varying AQP patterns likely play critical roles in the anatomical and physiological maturation of the salivary gland.
  • Potential functions include cell adhesion, migration, proliferation, apoptosis, transepithelial transport, osmosensing, and cell volume regulation.