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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Acrylic-based high internal phase emulsion polymeric monolith for capillary electrochromatography.

Yeliz Tunç¹, Ciğdem Gölgelioğlu, Nesrin Hasirci

¹Hacettepe University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, 06100 Ankara, Turkey.

Journal of Chromatography. A

|February 4, 2010

Summary

High internal phase emulsion polymers (polyHIPEs) show promise as stationary phases for capillary electrochromatography (CEC). These porous polyHIPEs enable efficient separation of alkylbenzenes with strong electroosmotic flow.

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Area of Science:

Analytical Chemistry
Polymer Science
Chromatography

Background:

High internal phase emulsion polymers (polyHIPEs) are largely unexplored for capillary electrochromatography (CEC) applications.
Existing literature on similar structures is limited, highlighting a gap in understanding their potential for CEC.

Purpose of the Study:

To introduce and evaluate polyHIPEs as a novel stationary phase for CEC.
To investigate the preparation and performance of polyHIPEs for separating alkylbenzenes.

Main Methods:

Preparation of polyHIPEs by in situ polymerization of isodecylacrylate (IDA) and divinylbenzene (DVB) within a high internal phase emulsion (HIPE).
Evaluation of polyHIPEs as monolithic columns in CEC for alkylbenzene separation.
Characterization of electroosmotic flow (EOF) and chromatographic performance.

Main Results:

Synthesized polyHIPEs exhibited high porosity and an interconnected open-cell structure.
The polyHIPEs demonstrated successful separation of alkylbenzenes, attributed to their well-defined porous structure.
A strong electroosmotic flow (EOF) was observed without specialized monomers, likely due to sulfate groups from the initiator.
Optimal separation performance was achieved using 70% acetonitrile (ACN) in the mobile phase, yielding high column efficiency (up to 200,000 plates/m).

Conclusions:

PolyHIPEs are effective and promising stationary phases for CEC applications.
The inherent porous structure and initiator-derived functional groups contribute to excellent chromatographic performance and EOF.
These findings open new avenues for utilizing polyHIPEs in advanced separation technologies.