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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Updated: Jun 16, 2026

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
08:47

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Published on: March 15, 2021

Biological tissues analysis by XRF microtomography.

G R Pereira1, H S Rocha, C Calza

  • 1Nuclear Instrumentation Laboratory (LIN), COPPE, UFRJ, Rio de Janeiro, Brazil.

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
|February 4, 2010
PubMed
Summary
This summary is machine-generated.

This study mapped elemental distribution in breast and prostate tissues using X-ray fluorescence microtomography (microCT). Findings correlate elemental concentrations with tissue characteristics and pathology.

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Area of Science:

  • Biomedical imaging
  • Materials science
  • Medical physics

Background:

  • Elemental distribution in tissues can be linked to specific pathologies.
  • Advanced imaging techniques are crucial for detailed tissue analysis.

Purpose of the Study:

  • To determine elemental distribution in breast and prostate tissues.
  • To correlate elemental concentrations with tissue characteristics and pathology using microCT.

Main Methods:

  • X-ray fluorescence microtomography (microCT) was employed.
  • Experiments were conducted at the Brazilian Synchrotron Light Laboratory.
  • Image reconstruction utilized filtered-back-projection with absorption corrections.

Main Results:

  • Elemental distribution patterns were identified in breast and prostate tissues.
  • Specific elemental concentrations were correlated with observed tissue characteristics and pathology.

Conclusions:

  • MicroCT is effective for visualizing elemental distribution in biological tissues.
  • Elemental mapping provides insights into tissue characteristics and disease states.