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Rat small intestinal mucins: a quantitative analysis.

A C Kemper1, R D Specian

  • 1Department of Cellular Biology and Anatomy, Louisiana State University Medical Center, Shreveport 71130.

The Anatomical Record
|February 1, 1991
PubMed
Summary
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This study quantifies neutral stored mucin in rat small intestine, revealing a proximal-to-distal increase. Pilocarpine treatment caused significant mucin depletion, validating the methodology for studying secretion.

Area of Science:

  • Gastroenterology
  • Histology
  • Cell Biology

Background:

  • Goblet cells secrete mucins in the small intestine.
  • Quantitative distribution of stored mucins is largely unknown.
  • Morphometric techniques can assess mucin storage and secretion.

Purpose of the Study:

  • Quantify neutral stored mucin distribution in the rat small intestine.
  • Establish morphometric methods to measure mucin.
  • Verify methodology by detecting pilocarpine-induced secretion.

Main Methods:

  • Vascular perfusion fixation of Wistar rats.
  • Intestine divided into 10 segments; sections stained with PAS and toluidine blue.
  • Morphometry using Merz grid and point-counting for mucin volume density.

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Main Results:

  • Stored mucin increased from proximal to distal small intestine, primarily in villi.
  • Pilocarpine induced massive mucin secretion, depleting crypt stores.
  • Villus mucin secretion occurred but was significant only in specific locations.

Conclusions:

  • Established a quantitative method for assessing small intestinal mucin distribution.
  • Demonstrated a proximal-to-distal gradient of stored mucin.
  • Validated the method's sensitivity to secretory stimuli.