Related Experiment Video
Updated: Jun 16, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
Toona sinensis extracts induced cell cycle arrest and apoptosis in the human lung large cell carcinoma
Cheng-Yuan Wang1, Kai-Huang Lin, Chih-Jen Yang
1Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan.
Abstract:
Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze-drying the supernatant of centrifuged crude extract from Toona sinensis leaves (TSL-1) to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3-(4-,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL-1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin-dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL-1 induced H661 cell apoptosis. Western blot analysis showed that TSL-1 reduced the expression of the anti-apoptotic protein B-cell lymphoma 2, and degraded the DNA repair protein, poly(ADP-ribose) polymerase. TSL-1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.
Insights
Toona sinensis leaf extract (TSL-1) demonstrated anti-cancer effects against human lung large cell carcinoma cells. TSL-1 inhibited cell proliferation and induced apoptosis, suggesting therapeutic potential.
Area of Science:
- Phytochemistry
- Oncology
- Molecular Biology
Background:
- Toona sinensis extracts show anti-cancer activity in various human cancer cell lines.
- The anti-cancer properties of Toona sinensis in human lung large cell carcinoma remain uninvestigated.
- Safety of Toona sinensis extracts has been established in animal studies.
Purpose of the Study:
- To investigate the anti-cancer effects of Toona sinensis leaf extract (TSL-1) on human lung large cell carcinoma (H661) cells.
- To elucidate the mechanisms underlying TSL-1's effects on H661 cell viability, cell cycle, and apoptosis.
Main Methods:
- Freeze-dried Toona sinensis leaf extract (TSL-1) was used to treat H661 cells.
- Cell viability was assessed using the MTT assay.
- Cell cycle progression and apoptosis were analyzed by flow cytometry and Western blotting.
Main Results:
- TSL-1 significantly reduced H661 cell viability.
- TSL-1 induced cell cycle arrest by downregulating cyclin-dependent kinase 4 and cyclin D1, and upregulating p27.
- TSL-1 promoted apoptosis by decreasing B-cell lymphoma 2 expression and degrading poly(ADP-ribose) polymerase.
Conclusions:
- TSL-1 exhibits significant anti-cancer properties against human lung large cell carcinoma.
- TSL-1 exerts its effects by modulating cell cycle regulatory proteins and inducing apoptosis.
- TSL-1 holds promise as a novel therapeutic agent or adjuvant for treating human lung large cell carcinoma.