Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon towards...
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caenorhabditis tropicalis followed a unique evolutionary path to self-fertility.

Genetics·2026
Same author

MUC4-Positive Fibroblastoma: Clinicopathological and Molecular Analysis of 7 Cases.

The American journal of surgical pathology·2026
Same author

Refining Tumor Mutational Burden as a Predictive Biomarker for Pembrolizumab: A Real-World Analysis in Japanese Patients.

Cancer science·2026
Same author

Intra-Articular and Juxta-Articular Lipomas of the Knee: Clinicopathologic Analysis of 18 Cases Highlighting Frequent Herniation and Characteristic Histology.

The American journal of surgical pathology·2026
Same author

Haldane's law works through X:Autosome incompatibility in Caenorhabditis briggsae/C. nigoni hybrids.

Nature communications·2026
Same author

The <i>Caenorhabditis</i> Gli protein TRA-1 makes a transcriptional activator that promotes spermatogenesis.

iScience·2025
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

SRC induces podoplanin expression to promote cell migration.

Yongquan Shen1, Chen-Shan Chen2, Hitoshi Ichikawa3

  • 1Molecular Biology Department, Stratford, New Jersey 08084.

The Journal of Biological Chemistry
|February 4, 2010
PubMed
Summary
This summary is machine-generated.

Normal cells can reverse cancer traits through contact normalization. This study reveals podoplanin (Pdpn) is key to tumor cell migration and is suppressed by normal cells, offering new cancer targets.

More Related Videos

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
07:43

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Published on: August 23, 2016

Related Experiment Videos

Last Updated: Jun 16, 2026

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
11:39

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

Published on: April 29, 2016

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
07:43

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Published on: August 23, 2016

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Nontransformed cells can revert tumor cells to a normal state via contact normalization.
  • Mechanisms of contact normalization, crucial for preventing cancer invasion, remain poorly understood.
  • Tumor cell migration is essential for malignancy and metastasis.

Purpose of the Study:

  • To identify genes regulated by contact normalization in Src-transformed cells.
  • To elucidate the role of Src signaling in tumor cell migration and invasion.
  • To understand how normal cells suppress tumor-promoting phenotypes.

Main Methods:

  • Gene expression analysis in Src-transformed and nontransformed cells.
  • Investigating the role of Crk-associated substrate (Cas) in Src signaling.
  • Analyzing podoplanin (Pdpn) expression and its impact on cell migration.

Main Results:

  • Src-transformed cells exhibit increased migration, partly due to induced podoplanin (Pdpn) expression.
  • Podoplanin (Pdpn) is identified as a key mediator of tumor cell migration induced by Src.
  • Nontransformed cells suppress Pdpn expression in adjacent Src-transformed cells, reversing migratory phenotypes.
  • Pdpn was among 23 genes upregulated by Src and downregulated by contact normalization.
  • 16 genes, including growth factor receptors, were downregulated by Src and upregulated by contact normalization.

Conclusions:

  • Podoplanin (Pdpn) is a critical mediator of Src-driven tumor cell migration and invasion.
  • Contact normalization by nontransformed cells suppresses Pdpn expression, inhibiting tumor cell migration.
  • Identification of Pdpn and other regulated genes provides potential therapeutic targets for cancer invasion and metastasis.