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Updated: Jun 16, 2026
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
Crystallization and preliminary crystallographic analysis of eukaryotic transcription and mRNA export factor Iws1
Michael Koch1, Marie Laure Diebold, Jean Cavarelli
1IGBMC (Institut de Génétique et Biologie Moléculaire et Cellulaire), Département de Biologie et Génomique Structurales, UDS, CNRS, INSERM, 1 Rue Laurent Fries, BP 10142, 67404 Illkirch CEDEX, France.
Abstract:
Transcription elongation by eukaryotic RNA polymerase II requires the coupling of mRNA synthesis and mRNA processing and export. The essential protein Iws1 is at the interface of these processes through its interaction with histone chaperone and elongation factor Spt6 as well as with complexes involved in mRNA processing and export. Upon crystallization of the evolutionarily conserved domain of Iws1 from Encephalitozoon cuniculi, four different crystal forms were obtained. Three of the crystal forms belonged to space group P2(1) and one belonged to space group P222(1). Preliminary X-ray crystallographic analysis of one of the crystal forms allowed the collection of data to 2.5 A resolution.
