Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

A Taylor series is a power series constructed to reproduce the local behavior of a smooth function about a chosen point, called the center. Its purpose is to encode the function’s value and successive derivatives into a structured expansion that captures local variation. The central task in its derivation is to find coefficients so that the series and the function share identical values and derivatives at the center.Consider a power series centered at x =...

The Taylor series provides a systematic method for approximating a smooth function by a polynomial that closely matches the function near a chosen point. This approach is particularly valuable in scientific and engineering contexts where functions may be difficult to evaluate directly, such as oscillatory voltages in alternating current (AC) circuits. Replacing complex functions with polynomial expressions simplifies computation while preserving essential local behavior. Taylor’s Theorem...

Taylor series provide a systematic way to represent a smooth function as an infinite polynomial centered at a chosen point. When that center is zero, the expansion is called a Maclaurin series. This form is especially useful because every derivative is evaluated at zero, which often makes the coefficients easier to compute. In applied mathematics and physics, such series are valuable because they replace complicated functions with polynomials that are easier to analyze and evaluate...

Signal processing techniques are essential for accurately converting continuous signals to digital formats and vice versa. When a continuous signal is sampled with a period T, the resulting sampled signal exhibits replicas of the original spectrum in the frequency domain, spaced at intervals equal to the sampling frequency. To handle this sampled signal, a zero-order hold method can be applied, which creates a piecewise constant signal by retaining each sample's value until the next sampling...

Managing signal sampling rates is essential in digital signal processing to maintain signal integrity. A decimated signal, characterized by a reduced frequency range due to its lower sampling rate, can be upsampled by inserting zeros between each sample. This upsampling process expands the original spectrum and introduces repeated spectral replicas at intervals dictated by the new Nyquist frequency. To refine this zero-inserted sequence, it is passed through a lowpass filter with a cutoff...