Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Excitation of an optical fiber by a Gaussian beam.

Applied optics·2010
Same author

Rate of vascularization of coralline hydroxyapatite spherical implants pretreated with saline/gentamicin, rTGF-beta 2, and autogenous plasma.

Ophthalmic plastic and reconstructive surgery·1998
Same author

Focused array hyperthermia applicator: theory and experiment.

IEEE transactions on bio-medical engineering·1984
Same author

Digital imaging of gamma-ray sources with depth information.

IEEE transactions on bio-medical engineering·1976

Related Experiment Video

Updated: Jun 16, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Digital and optical reconstruction of images from suboptical diffraction patterns.

T H Demetrakopoulos, R Mittra

    Applied Optics
    |February 4, 2010
    PubMed
    Summary

    This study explores digital and optical reconstruction of synthetic holograms. It details methods for digital reconstruction using inverse diffraction transforms and image enhancement, and addresses longitudinal distortion in optical reconstruction.

    More Related Videos

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

    Published on: February 8, 2014

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    Area of Science:

    • Holography
    • Digital Image Processing
    • Optical Reconstruction

    Background:

    • Synthetic holograms are crucial for advanced imaging.
    • Suboptical frequencies present unique reconstruction challenges.
    • Longitudinal distortion is a known issue in optical reconstruction.

    Purpose of the Study:

    • To investigate digital reconstruction of suboptical frequency synthetic holograms.
    • To explore methods for mitigating longitudinal distortion in optical reconstruction.
    • To compare the advantages and disadvantages of digital versus optical reconstruction schemes.

    Main Methods:

    • Digital reconstruction using inverse diffraction transform on diffraction patterns.
    • Sequential display of object cross-sections on a CRT.
    • Application of image enhancement techniques during digital reconstruction.
    • Method development for reducing longitudinal distortion in optical reconstruction.
    • Analysis of diffuse and nondiffuse illumination schemes.

    Main Results:

    • Digital reconstruction successfully displays object cross-sections.
    • Image enhancement improves digital reconstruction quality.
    • A method is proposed to partially correct longitudinal distortion in optical reconstruction.
    • Both digital and optical schemes have distinct benefits and drawbacks.

    Conclusions:

    • Digital reconstruction offers a viable alternative for synthetic holograms.
    • Techniques exist to improve optical reconstruction fidelity.
    • The choice between digital and optical reconstruction depends on specific application needs.