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E. coli K-12 and EHEC genes regulated by SdiA.

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Escherichia coli SdiA (a LuxR family transcription factor) regulates different genes when expressed from a plasmid versus its natural chromosomal location. True AHL-mediated gene regulation in E. coli differs from previous plasmid-based studies.

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Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Quorum Sensing

Background:

  • Escherichia and Salmonella possess SdiA, a LuxR family transcription factor.
  • SdiA regulates genes in response to N-acyl homoserine lactones (AHLs) from other bacteria.
  • Previous studies identified E. coli genes responding to plasmid-encoded sdiA, but not chromosomal sdiA or AHLs.

Purpose of the Study:

  • To identify genes regulated by chromosomal SdiA and/or AHLs in E. coli.
  • To clarify the discrepancies in gene regulation observed with plasmid-based versus chromosomal sdiA expression.

Main Methods:

  • Screened 20,000 random transposon-luciferase fusions in E. coli K-12 and O157:H7 for AHL response.
  • Tested identified genes for dependence on chromosomal sdiA.
  • Validated findings by assessing response to plasmid-based sdiA expression.

Main Results:

  • Genes ftsQAZ and acrAB do not respond to chromosomal sdiA or AHLs.
  • Chromosomal sdiA up-regulates glutamate-dependent acid resistance genes and down-regulates fliE.
  • SdiA-mediated gene regulation in E. coli is only partially dependent on AHLs.

Conclusions:

  • Genes responding to plasmid-based sdiA differ significantly from those regulated by chromosomal sdiA and/or AHLs.
  • This highlights the importance of studying sdiA in its native context for understanding AHL detection in E. coli.