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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Boronic acid-modified DNA that changes fluorescent properties upon carbohydrate binding.

Xiaochuan Yang1, Chaofeng Dai, Angie Dayan Calderon Molina

  • 1Department of Chemistry and Center for Biotechnology and Drug Design, Georgia State University, Atlanta, Georgia 30302-4098, USA.

Chemical Communications (Cambridge, England)
|February 4, 2010
PubMed
Summary

A novel boronic acid-modified TTP (NB-TTP) was synthesized and integrated into DNA. This DNA exhibits fluorescence changes when carbohydrates are added, enabling new detection methods.

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Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Molecular Biology

Background:

  • Boronic acids are versatile chemical moieties used in sensing applications.
  • DNA nanotechnology offers a platform for developing novel biosensors.
  • Developing specific and sensitive carbohydrate detection methods remains a challenge.

Purpose of the Study:

  • To synthesize a long-wavelength boronic acid-modified TTP (NB-TTP).
  • To enzymatically incorporate NB-TTP into DNA strands.
  • To investigate the fluorescent properties of NB-TTP modified DNA in response to carbohydrate binding.

Main Methods:

  • Chemical synthesis of NB-TTP.
  • Enzymatic DNA synthesis and modification.
  • Spectroscopic analysis of fluorescence changes.

Main Results:

  • Successful synthesis and enzymatic incorporation of NB-TTP into DNA.
  • Demonstrated intrinsic fluorescent changes in NB-TTP modified DNA upon carbohydrate addition.
  • The DNA probe exhibits selective fluorescence response to specific carbohydrates.

Conclusions:

  • NB-TTP is a suitable building block for creating fluorescent DNA-based carbohydrate sensors.
  • Enzymatic incorporation allows for straightforward generation of modified DNA probes.
  • This approach provides a novel strategy for carbohydrate detection with potential applications in diagnostics and research.