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Crypt abscess-associated microbiota in inflammatory bowel disease and acute self-limited colitis
Harry Sokol1, Nadia Vasquez, Nadia Hoyeau-Idrissi
1Gastroenterology and Nutrition Department, Saint Antoine Hospital, Pierre et Marie Curie-Paris6 University, AP-HP, 184 rue du Faubourg Saint-Antoine, 75571, Paris, France. sokol_harry@yahoo.fr
Aim:
To evaluate whether crypt abscesses from inflammatory bowel disease (IBD) patients contain bacteria and to establish their nature.
Methods:
We studied 17 ulcerative colitis patients, 11 Crohn's disease patients, 7 patients with acute self-limited colitis (ASLC) and normal colonic biopsies from 5 subjects who underwent colonoscopy for colon cancer screening. A fluorescent in situ hybridization technique was applied to colonic biopsies to assess the microbiota composition of the crypts and crypt abscesses.
Results:
Crypts colonized by bacteria were observed in 42.9% and 3.6% of ASLC and IBD patients, respectively (P = 0.019). Crypt abscesses colonized by bacteria were observed in 28.6% and 0.0% of ASLC and IBD patients, respectively (P = 0.035).
Conclusion:
These results do not support the hypothesis that crypt abscesses in IBD are the result of localized dysbiosis arising from persistence of living bacteria colonizing the crypts.
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