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Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry
J R Lakowicz1, J Kuśba, W Wiczk
1University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
Biophysical Chemistry
|January 1, 1991
Abstract:
We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.