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Related Experiment Video

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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

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Published on: May 21, 2012

Th-1/Th-2 cytokine pattern in human amoebic colitis.

A Rafiei¹, A Ajami, M Hajilooi

¹Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Pakistan Journal of Biological Sciences : PJBS

|February 5, 2010

Summary

Amoebiasis patients show elevated IL-5 and IL-13 cytokines, indicating a Th-2 immune response. This suggests a potential link between specific cytokine profiles and amoebic colitis development.

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Published on: December 16, 2021

Area of Science:

Immunology
Infectious Diseases
Gastroenterology

Background:

Amoebiasis, caused by Entamoeba histolytica, remains a significant public health issue, particularly in developing nations.
The precise immune response in human amoebiasis, especially amoebic colitis, is not fully understood.
Understanding host immune responses is crucial for developing effective therapeutic strategies.

Purpose of the Study:

To investigate and define the immune response in patients with amoebic colitis by evaluating key cytokine profiles.
To compare cytokine levels in patients with amoebic colitis against matched healthy controls.
To explore potential gender-specific differences in cytokine production during amoebiasis.

Main Methods:

A case-control association study involving 31 patients with amoebic colitis and 31 healthy controls.
Serum samples were analyzed for levels of Interleukin-12 (IL-12), Interferon-gamma (IFN-gamma), Interleukin-13 (IL-13), and Interleukin-5 (IL-5).
Cytokine quantification was performed using a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA).

Main Results:

Serum levels of IL-12, IFN-gamma, IL-13, and IL-5 were generally higher in patients with amoebic colitis compared to controls.
A statistically significant increase was observed in IL-5 (p = 0.04) and IL-13 (p = 0.014) levels in patients.
Elevated IL-13 levels were significantly higher in male patients compared to female patients (p = 0.04).

Conclusions:

Entamoeba histolytica infection induces a mixed Th-1/Th-2 immune response.
The findings suggest a polarization towards a Th-2 response during the early stages of amoebiasis.
This Th-2 dominant response may contribute to the clinical manifestation and progression of amoebic colitis.