Related Experiment Video
Updated: Jun 16, 2026

Calcium Carbonate Formation in the Presence of Biopolymeric Additives
Published on: May 14, 2019
Calcium carbonate-hydrolyzed soy protein complexation in the presence of citric acid
Latha-Selvi Canabady-Rochelle1, Christian Sanchez, Michel Mellema
1Nancy-Université, Institut National Polytechnique de Lorraine, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, Laboratoire d'Ingénierie des Biomolécules, 2, Avenue de la forêt de Hayes, 54505 Vandoeuvre-Lès-Nancy, France. l.canabady@voila.fr
Abstract:
The influence of hydrolyzed soy proteins on calcium carbonate stabilization was studied in citric acid solution. Calcium-soy proteins interactions were characterized using a calcium ion selective electrode, turbidity, and Isothermal Titration Calorimetry. Once the meta-stable phase was reached or just after soy protein addition, spray-drying was performed and SEM, XRD, and XPS analysis were carried out on spray-dried powders. In citric acid solution calcite crystals were eroded giving rise to smaller amorphous particles. In the presence of soy proteins, complexation exothermic in nature occurred with the mineral phase, which prevented CaCO(3) from recrystallisation and kept the system in an amorphous state. SEM performed on spray-dried powder showed that soy proteins were swollen in presence of mineral phase and resulted in a decrease of calcium concentration at the extreme surface of the studied powders as demonstrated by XPS.
More Related Videos
Related Concept Videos
Factors Affecting Solubility
Hydration of Cement
Titration of Polyprotic Base with a Strong Acid
Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
EDTA: Auxiliary Complexing Reagents
Polyprotic Acids

