Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
07:12

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Published on: November 24, 2010

Sindbis virus: propagation, quantification, and storage.

Raquel Hernandez1, Christine Sinodis, Dennis T Brown

  • 1North Carolina State University, Raleigh, North Carolina.

Current Protocols in Microbiology
|February 5, 2010
PubMed
Summary

Sindbis virus, a model Alphavirus, efficiently replicates in both insect and vertebrate cells. Its broad host range and genetic manipulability make it a valuable tool for studying virus-host interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does Neuroglobin Protect Against Stroke? Insights Into the Role of Neurovascular Unit Cells.

Cellular and molecular neurobiology·2026
Same author

Superinfection interference of alpha and orthoflavivirus pathogens by homologous non-pathogenic vaccine strains.

Microbiology spectrum·2025
Same author

The Interplay Between Melatonin and Nitric Oxide: Mechanisms and Implications in Stroke Pathophysiology.

Antioxidants (Basel, Switzerland)·2025
Same author

Nutraceutical Supplementation as a Potential Non-Drug Treatment for Fibromyalgia: Effects on Lipid Profile, Oxidative Status, and Quality of Life.

International journal of molecular sciences·2024
Same author

The Mediating Role of Exercise in Depression and Fatigue in Patients with Advanced Cancer.

Current oncology (Toronto, Ont.)·2024
Same author

Is there a preferred platinum and fluoropyrimidine regimen for advanced HER2-negative esophagogastric adenocarcinoma? Insights from 1293 patients in AGAMENON-SEOM registry.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2024

Area of Science:

  • Virology
  • Molecular Biology
  • Arbovirology

Background:

  • Sindbis virus, a prototype Alphavirus, exhibits a wide host range, necessitating replication in divergent invertebrate and vertebrate hosts.
  • The virus plays a crucial role in a complex transmission cycle involving insect vectors and vertebrate hosts.
  • Understanding Sindbis virus replication is key to comprehending arbovirus transmission dynamics.

Purpose of the Study:

  • To highlight the adaptability of Sindbis virus to diverse biological systems.
  • To underscore the utility of Sindbis virus as a model organism in scientific research.
  • To detail the resources and methods available for Sindbis virus research.

Main Methods:

  • Culturing Sindbis virus in various vertebrate and invertebrate cell lines.

More Related Videos

Virus Propagation and Cell-Based Colorimetric Quantification
07:26

Virus Propagation and Cell-Based Colorimetric Quantification

Published on: April 7, 2023

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
07:12

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Published on: November 24, 2010

Virus Propagation and Cell-Based Colorimetric Quantification
07:26

Virus Propagation and Cell-Based Colorimetric Quantification

Published on: April 7, 2023

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

  • Titration of infectious Sindbis virus on mammalian and mosquito cell lines.
  • Genetic manipulation using full-length cDNA clones and infectious RNA synthesis.
  • Transfection of cells via electroporation for mutant expression.

    • Main Results:

    • Sindbis virus achieves high replication titers in a broad spectrum of cultured cells from both invertebrate and vertebrate origins.
    • Established protocols allow for straightforward infectivity titration on multiple cell types.
    • Availability of cDNA clones facilitates genetic engineering of the virus.
    • Electroporation enables efficient expression of viral RNA mutants in selected cell lines.

    Conclusions:

    • Sindbis virus's broad host range and high replication efficiency in diverse cells are key characteristics.
    • The availability of genetic tools and established cell culture systems makes Sindbis virus an amenable model for virological studies.
    • Further research utilizing Sindbis virus can advance our understanding of Alphavirus replication and host interactions.