Related Experiment Video
Updated: Jun 16, 2026

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
Published on: June 7, 2017
Antimicrobial efficacy of soap and water hand washing versus an alcohol-based hand cleanser
Ronald H Holton1, Michaell A Huber, Geza T Terezhalmy
1Clinical Laboratory Science, The University of Texas Health Science Center at San Antonio Dental School, San Antonio, Texas, USA.
Abstract:
The emergence of alcohol-based hand cleansers may represent an alternative to soap and water in the clinical dental setting. In this study, the antimicrobial efficacy of traditional hand washing vs. a unique alcohol-based hand cleanser with persistence was evaluated. Two experienced dentists participated over a 10-day period. On days 1-5, each clinician used an antibacterial liquid soap (Dial, Dial Corporation, Scottsdale, AZ). Days 6-10, an alcohol-based hand cleanser (Triseptin Water Optional, Healthpoint Surgical, Fort Worth, TX) was used. Sampling was by modified glove juice technique. The results indicate that the alcohol-based hand cleanser dramatically outperforms the traditional hand washing agent in the general dental setting.
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Hand wash basins in clinical areas should have faucets that can be turned on and off without using the hands; that is, they should be non-touch or lever-operated.
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail paint...
Handwashing III: During the Procedure and Post-Procedure Steps
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