Detection of citrus Huanglongbing by Fourier transform infrared-attenuated total reflection spectroscopy
Samantha A Hawkins1, Bosoon Park, Gavin H Poole
1US Department of Agriculture-Agricultural Research Service Russell Research Center, 950 College Station Rd, Athens, Georgia 30605, USA. samantha.hawkins@ars.usda.gov
Abstract:
Citrus Huanglongbing (HLB, also known as citrus greening disease) was discovered in Florida in 2005 and is spreading rapidly amongst the citrus growing regions of the state. Detection via visual symptoms of the disease is not a long-term viable option. New techniques are being developed to test for the disease in its earlier presymptomatic stages. Fourier transform infrared-attenuated total reflection (FT-IR-ATR) spectroscopy is a candidate for rapid, inexpensive, early detection of the disease. The mid-infrared region of the spectrum reveals dramatic changes that take place in the infected leaves when compared to healthy non-infected leaves. The carbohydrates that give rise to peaks in the 900-1180 cm(-1) range are reliable in distinguishing leaves from infected plants versus non-infected plants. A model based on chemometrics was developed using the spectra from 179 plants of known disease status. This model then correctly predicted the status of >95% of the plants tested.
More Related Videos
Related Concept Videos
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
IR Frequency Region: Fingerprint Region
The...
IR Spectrometers
IR Spectrum
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...
Infrared (IR) Spectroscopy: Overview
Different compounds display unique properties due to their...
Total Internal Reflection Fluorescence Microscopy


