Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.

D Gems1, I L Johnstone, A J Clutterbuck

  • 1Institute of Genetics, University of Glasgow, Scotland, U.K.

Gene
|February 1, 1991
PubMed
Summary

Researchers discovered a new plasmid, ARp1, that significantly enhances gene transformation in Aspergillus species. This novel vector offers a 250-fold improvement in transformation efficiency for Aspergillus nidulans, A. niger, and A. oryzae.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

When and How Can Death Be an Adaptation?

Biochemistry. Biokhimiia·2019
Same author

Heat ray cataract.

Transactions. Ophthalmological Society of the United Kingdom·2010
Same author

SODIUM EVIPAN ANAESTHESIA IN OPHTHALMIC SURGERY: WITH SPECIAL REFERENCE TO DISADVANTAGES.

British medical journal·2010
Same author

SWALLOWING FOREIGN BODIES FOR A LIVELIHOOD.

British medical journal·2010
Same author

Sulphonamide Treatment of Hypopyon Ulcer of the Cornea.

British medical journal·2010
Same author

Studies on fly strike in Merino sheep; a surgical operation for the control of tail strike.

Australian veterinary journal·2010

Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • Gene transformation is crucial for genetic studies in filamentous fungi like Aspergillus.
  • Existing integrative vectors have limitations in transformation efficiency.
  • Development of efficient extrachromosomal vectors is needed for Aspergillus research.

Purpose of the Study:

  • To isolate and characterize a novel plasmid for enhanced gene transformation in Aspergillus species.
  • To evaluate the transformation efficiency and stability of the novel plasmid.

Main Methods:

  • Transformation of Aspergillus nidulans with a gene bank.
  • Reisolation and characterization of the plasmid ARp1.
  • Southern blot analysis to determine plasmid integration and copy number.

Related Experiment Videos

  • Mitotic stability assays.

    • Main Results:

    • A plasmid, ARp1 (11.5 kb), was reisolated with a 250-fold higher transformation efficiency in A. nidulans compared to integrative vectors.
    • ARp1 exists as an extrachromosomal element with a copy number of 10-30 per haploid genome.
    • The plasmid is mitotically unstable, lost in 65% of progeny.
    • ARp1 demonstrated similar transformation properties in A. niger and A. oryzae.

    Conclusions:

    • The novel plasmid ARp1 significantly enhances Aspergillus transformation efficiency.
    • ARp1 functions as an extrachromosomal element, suitable for applications requiring transient gene expression or selection.
    • ARp1's broad host range makes it a valuable tool for genetic manipulation across multiple Aspergillus species.