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Related Experiment Video

Updated: Jun 16, 2026

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Published on: January 22, 2015

Method development for electrotransformation of Acidithiobacillus caldus.

Linxu Chen¹, Jianqun Lin, Bing Li

¹State key Lab of Microbial Technology, Shandong University, Jinan 250100, China.

Journal of Microbiology and Biotechnology

|February 6, 2010

Summary

Electrotransformation successfully introduced foreign genes into Acidithiobacillus caldus (A. caldus), overcoming challenges with previous methods. This new technique enhances genetic manipulation for this important bioleaching bacterium.

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Area of Science:

Microbiology
Molecular Biology
Biotechnology

Background:

Acidithiobacillus caldus (A. caldus) is an acidophilic, chemoautotrophic bacterium crucial for bioleaching processes.
Genetic manipulation of A. caldus is challenging, with conjugation being the only previously successful, albeit complex, method.

Purpose of the Study:

To develop and optimize a novel electrotransformation method for efficient gene introduction into A. caldus.
To establish a convenient alternative to existing genetic transformation techniques for A. caldus.

Main Methods:

Electrotransformation of A. caldus Y-3 using plasmid pJRD215.
Confirmation of transformants via colony PCR targeting the str gene.
Plasmid recovery from putative transformants to validate successful transformation.

Main Results:

Successful electrotransformation of A. caldus was achieved for the first time.
Transformation efficiency was significantly improved, increasing from 0.8 to 3.6 x 10^4 transformants per microgram of plasmid DNA.
The developed method proved convenient for introducing foreign genes.

Conclusions:

Electrotransformation provides an effective and accessible method for genetic engineering of A. caldus.
This advancement facilitates further research and application of A. caldus in bioleaching and other industrial processes.