Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effective and evidence-based management strategies for rosacea: summary of a Cochrane systematic review.

The British journal of dermatology·2011
Same author

On using time-averaging restraints in molecular dynamics simulation.

Journal of biomolecular NMR·2009
Same author

Cutaneous reaction to pegfilgrastim presenting as severe generalized skin eruption.

The British journal of dermatology·2009
Same author

Interventions for rosacea.

The Cochrane database of systematic reviews·2005
Same author

Autonomy, consent, and limiting healthcare costs.

Journal of medical ethics·2005
Same author

The TGF-beta 1 gene codon 10 polymorphism contributes to the genetic predisposition to nephropathy in Type 1 diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2004
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
06:50

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

Published on: December 2, 2017

Sensitive technique for measuring differences in reflectivity.

W R Scott, L Muldawer, M A Graber

    Applied Optics
    |February 6, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel reflectometer technique uses a tuning fork and lock-in amplifier to precisely measure reflectivity differences between samples. This method offers high sensitivity for detecting subtle changes in material reflectivity.

    More Related Videos

    A Stable Phantom Material for Optical and Acoustic Imaging
    04:54

    A Stable Phantom Material for Optical and Acoustic Imaging

    Published on: June 16, 2023

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
    06:50

    Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

    Published on: December 2, 2017

    A Stable Phantom Material for Optical and Acoustic Imaging
    04:54

    A Stable Phantom Material for Optical and Acoustic Imaging

    Published on: June 16, 2023

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    Area of Science:

    • Physics
    • Materials Science
    • Optical Metrology

    Background:

    • Accurate measurement of sample reflectivity is crucial in various scientific fields.
    • Existing techniques may have limitations in speed, sensitivity, or operational environment.

    Purpose of the Study:

    • To introduce a new, highly sensitive technique for measuring reflectivity differences between two samples.
    • To demonstrate the advantages of the developed system for optical measurements.

    Main Methods:

    • Utilizing an electrically driven tuning fork to alternately position samples within a reflectometer beam.
    • Employing a lock-in amplifier for sensitive detection of the differential reflectivity signal.

    Main Results:

    • The developed system achieves high sensitivity, capable of detecting reflectivity changes as small as 0.001%.
    • The technique offers advantages such as short deadtime and suitability for vacuum operation.

    Conclusions:

    • The new reflectometer technique provides a sensitive and efficient method for comparative reflectivity measurements.
    • This advancement has potential applications in materials characterization and optical device analysis.