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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Analysis of the degradome with the CLIP-CHIP microarray.

Reinhild Kappelhoff1, Ulrich Auf dem Keller, Christopher M Overall

  • 1Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 6, 2010
PubMed
Summary

The CLIP-CHIP microarray analyzes protease and inhibitor gene transcripts in humans and mice at the mRNA level. This tool provides expression profiles for differential gene expression analysis.

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Area of Science:

  • Biochemistry
  • Genomics
  • Molecular Biology

Background:

  • Proteases and inhibitors play crucial roles in biological processes.
  • Understanding their expression patterns is vital for disease research.
  • Existing methods may not offer a comprehensive analysis of these gene families.

Purpose of the Study:

  • To introduce the CLIP-CHIP, a novel microarray for analyzing protease and inhibitor gene transcripts.
  • To enable the study of differential gene expression in human and murine genomes.

Main Methods:

  • Development of the CLIP-CHIP microarray using unique 70-mer oligonucleotides.
  • Design of oligonucleotides to match known or predicted protease and inhibitor mRNA sequences.
  • Printing of oligonucleotides on a glass-matrix surface for high-throughput analysis.

Main Results:

  • The CLIP-CHIP allows for the simultaneous analysis of all protease, non-proteolytic homolog, and protease inhibitor gene transcripts.
  • It provides expression profiles at the mRNA transcript level for human and murine samples.
  • The microarray facilitates the identification of differentially expressed genes within these families.

Conclusions:

  • The CLIP-CHIP is a powerful tool for comprehensive degradome analysis.
  • It enables detailed investigation of protease and inhibitor gene expression.
  • This technology aids in understanding gene expression profiles in various biological contexts.