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Related Concept Videos

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Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
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Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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Published on: April 29, 2022

Mechanism-based profiling of MMPs.

Jed F Fisher1, Shahriar Mobashery

  • 1Department of Chemistry and Biochemistry, Walther Cancer Research Center, University of Notre Dame, Notre Dame, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 6, 2010
PubMed
Summary
This summary is machine-generated.

Developing sensitive assays for matrix metalloproteinase (MMP) activity is crucial for correlating MMP levels with disease progression. New methods enable quantitative and qualitative profiling of MMP activity in cells and tissues for targeted therapeutic strategies.

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Last Updated: Jun 16, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Successful clinical use of matrix metalloproteinase (MMP) inhibitors necessitates quantitative correlation with disease type and progression.
  • Intensive research efforts are focused on developing sensitive assay methods for MMP activity.
  • Improved analytical methods are needed for MMP sub-type inhibition profiling and activity determination.

Purpose of the Study:

  • To review recent progress in quantitative and qualitative profiling of MMP activity in cells and tissues.
  • To highlight advancements in MMP assay development for clinical applications.
  • To emphasize the importance of MMP sub-type-specific analysis.

Main Methods:

  • Quantitative determination of MMP activity using broad-spectrum MMP inhibitors immobilized on chromatography resin to retain active MMP.
  • Qualitative determination of MMP involvement in disease using MMP sub-type-selective inhibitors.
  • Characterization of eluted active MMP sub-types through appropriate analysis.

Main Results:

  • A method for quantitative MMP activity determination by selectively retaining active MMPs.
  • Proof of principle for qualitative determination using thiirane-based MMP inhibitors (e.g., SB-3CT) for MMP-2 and MMP-9.
  • Demonstration of MMP inhibitor efficacy in disease models dependent on MMP-2/-9, but not in models involving other MMPs like MMP-14.

Conclusions:

  • Accurate quantitative and qualitative profiling of MMP activity is essential for effective MMP inhibitor-based therapies.
  • Selective MMP inhibitors show promise in treating diseases driven by specific MMP sub-types.
  • The developed methods facilitate a deeper understanding of MMP roles in disease pathogenesis and progression.