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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

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Related Experiment Video

Updated: Jun 16, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

siRNA applications in nanomedicine.

Talar Tokatlian1, Tatiana Segura

  • 1University of California, Los Angeles, CA 90095, USA.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|February 6, 2010
PubMed
Summary

RNA interference (RNAi) offers therapeutic potential, but effective delivery of small interfering RNA (siRNA) is challenging. This review explores nanoparticle strategies to overcome delivery barriers and enhance siRNA therapeutics for in vivo applications.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Delivery

Background:

  • RNA interference (RNAi) is a sequence-specific gene silencing mechanism utilizing small interfering RNA (siRNA).
  • siRNA is processed by the RNA-induced silencing complex (RISC) for mRNA targeting and cleavage.
  • Effective delivery of siRNA therapeutics is a significant challenge in clinical applications.

Purpose of the Study:

  • To review recent advances in siRNA delivery strategies for therapeutic applications.
  • To highlight delivery approaches that have demonstrated in vivo success or novel functionality.
  • To discuss barriers to efficient siRNA intracellular trafficking and cellular targeting.

Main Methods:

  • Discussion of various siRNA delivery barriers.
  • Categorization of delivery approaches by size: direct siRNA modification (<10 nm), self-assembled particles (100-300 nm), neutral liposomes (<200 nm), and macroscale matrices (>100 microm).

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Published on: March 25, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
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Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

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Last Updated: Jun 16, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

  • Focus on strategies with in vivo success or enhanced intracellular trafficking/targeting capabilities.

    • Main Results:

    • Nanoparticle-based strategies are being developed to enhance siRNA drug delivery.
    • Different nanoparticle sizes and compositions (cationic polymers, lipids, liposomes) are employed.
    • Novel functionalities are being introduced for improved cellular uptake and targeting.

    Conclusions:

    • Nanoparticle design is crucial for overcoming siRNA delivery limitations.
    • Advances in delivery systems show promise for in vivo therapeutic applications of RNAi.
    • Continued research in nanotechnology is vital for realizing the full therapeutic potential of siRNA.