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Formation of factor 390 by cell extracts of Methanosarcina barkeri
W M van de Wijngaard1, P vermey, C van der Drift
1Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
Journal of Bacteriology
|April 1, 1991
Abstract:
Cell extracts of Methanosarcina barkeri converted coenzyme F420 in an ATP-dependent reaction to the adenylylated derivative factor 390. Although it was reported previously (L. M. Gloss and R. P. Hausinger, BioFactors 1:237-240, 1988) that whole cells were unable to perform this conversion, we observed the conversion in 7 of 11 extracts, all of which were prepared from different batches of cells.