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Published on: November 1, 2015
A quick procedure for identifying doubly homozygous immunodeficient scid beige mice
1Laboratoire d'Immunologie, Université Louis Pasteur Strasbourg, Illkirch, France.
Journal of Immunological Methods
|March 21, 1991
Summary
Researchers developed a method to identify double mutant mice lacking T, B, and NK cells. These immunodeficient mice are crucial for studying diseases and potential therapies.
Area of Science:
- Immunology
- Genetics
- Animal Models
Background:
- Severe Combined Immunodeficiency (SCID) mice lack T and B lymphocytes.
- Beige (bg) mice exhibit reduced Natural Killer (NK) cell activity.
- Combining these deficiencies creates a more profound immunodeficiency.
Purpose of the Study:
- To establish a rapid and reliable method for identifying mice with combined SCID and beige mutations.
- To characterize the phenotype of these double mutant mice.
- To assess their utility as recipients for xenografts.
Main Methods:
- Crossbreeding CB17 scid and B6 bg mice.
- Identifying double homozygous [scid, bg] progeny in the F2 generation.
- Monitoring hypogammaglobulinemia (SCID) and prolonged bleeding (beige).
- Grafting splenocytes and bone marrow cells from F1 mice to protect against infection.
Main Results:
- A procedure was established to identify [scid, bg] mice.
- These mice exhibit combined SCID and beige immunodeficiencies.
- They show high susceptibility to infections, requiring protective cell grafts.
- The [scid, bg] mice are proposed as improved xenograft recipients.
Conclusions:
- The developed method allows for efficient identification of [scid, bg] mice.
- [scid, bg] mice possess a unique immunodeficient phenotype.
- These mice offer potential advantages for xenograft research over traditional SCID models.
