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Updated: Jun 16, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
[Study on the automatic ribotyping for Enterobacter sakazakii]
Xiao-yan Pei1, Yun-chang Guo, Xiu-mei Liu
1National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
Objective:
To analyze the ribotyping fingerprint of Enterobacter sakazakii (E. sakazakii) isolated from food and its typing power.
Methods:
Two standard strains and twenty-eight isolates of E.sakazakii were analyzed by the DuPont Riboprinter(TM) microbial characterization system. The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software.
Results:
This system grouped two standard strains and twenty-eight E.sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively, the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters.
Conclusion:
The automatic ribotyping method is convenient and fast in E.sakazakii typing.
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