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Updated: Jun 16, 2026
07:26
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
[The sonic hedgehog induce vascular adventitial fibroblasts phenotypic modulation, proliferation and migration]
Feng-he Li1, Shi-jie Xin, Si-yang Zhang
1Department of Vascular Surgery, First Affiliated Hospital, China Medical University, Shenyang, China.
Zhonghua Yi Xue Za Zhi
|February 9, 2010
Summary
Sonic hedgehog (shh) signaling promotes vascular adventitial fibroblast proliferation and migration. This study demonstrates shh
Area of Science:
- Vascular biology
- Cell signaling
- Fibroblast research
Context:
- Vascular adventitial fibroblasts play a role in vascular remodeling.
- The sonic hedgehog (shh) signaling pathway is implicated in various cellular processes.
Purpose:
- To investigate the impact of shh on vascular adventitial fibroblast behavior.
- To assess shh's role in fibroblast migration, proliferation, and phenotypic modulation.
Summary:
- Sonic hedgehog (shh) was detected in vascular adventitial fibroblasts in vitro.
- Exogenous shh increased Ki67-positive cells and wound closure, indicating enhanced proliferation and migration.
- Cyclopamine treatment reduced Ki67-positive cells and wound closure, confirming shh's pro-proliferative and pro-migratory effects.
- Alpha-actin expression was observed, suggesting phenotypic modulation.
Impact:
- Shh signaling is a key regulator of vascular adventitial fibroblast activity.
- Findings suggest shh as a potential therapeutic target in conditions involving vascular remodeling.
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