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Yellow Fever

Yellow Fever

Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...

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Related Experiment Video

Updated: Jun 16, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Efficient, trans-complementing packaging systems for chimeric, pseudoinfectious dengue 2/yellow fever viruses.

Alexandr V Shustov¹, Ilya Frolov

¹Department of Microbiology, BBRB 373/Box 3, University of Alabama, Birmingham, AL 35294-2170, USA.

|February 9, 2010

Summary

This summary is machine-generated.

Researchers developed novel pseudoinfectious flaviviruses (PIVs) as vaccine candidates, combining live vaccine efficiency with safety. These PIVs encode dengue virus 2 glycoproteins using yellow fever virus machinery, producing safe, noninfectious particles.

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Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Published on: April 8, 2012

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Last Updated: Jun 16, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Published on: April 8, 2012

Area of Science:

Virology
Vaccinology
Molecular Biology

Background:

Pseudoinfectious viruses (PIVs) offer a promising vaccine strategy by merging live vaccine efficacy with enhanced safety.
Previous work established a framework for developing PIVs as vaccine candidates.

Purpose of the Study:

To further develop chimeric PIVs for use as vaccine candidates.
To engineer PIVs encoding dengue virus 2 (DEN2V) glycoproteins with yellow fever virus (YFV)-derived replicative machinery.

Main Methods:

Introduction of synergistic mutations in prM and NS2A proteins to abolish processing.
Utilizing NS1-deficient YFV helper RNAs for in vitro packaging of PIV genomes into infectious virions.
Passaging PIVs and helper RNAs as two-component genome viruses.

Main Results:

Successfully created defective, pseudoinfectious flaviviruses (PIVs) encoding DEN2V glycoproteins and YFV replicons.
Engineered PIVs produce noninfectious, immature, and/or subviral DEN2V particles due to mutations.
Achieved high-titer packaging of PIV genomes using helper RNAs, enabling scalable propagation.

Conclusions:

The developed chimeric PIVs represent advanced vaccine candidates with a strong safety profile.
The two-component genome system allows for controlled replication and large-scale production.
This strategy holds potential for developing novel vaccines against flaviviruses like dengue.