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A competitive indirect enzyme-linked immunoassay for lead ion measurement using mAbs against the lead-DTPA complex

Jun-jian Xiang1, Yi-fan Zhai, Yong Tang

  • 1Molecular Immunology and Antibody Engineering Center, Jinan University, Guangzhou 510632, China.

Environmental Pollution (Barking, Essex : 1987)
|February 9, 2010
PubMed

Immunoassays for quantitative measurement of environmental heavy metals offer several advantages over other traditional methods. To develop an immunoassay for lead, Balb/c mice were immunized with a lead-chelate-protein conjugate to allow maximum exposure of the metal to the immune system. Three stable hybridoma cell lines were obtained through spleen cells fusion with Sp2/0 cells. One cell line, 2A11D11, produced mAbs with preferential selectivity and sensitivity for Pb-DTPA than DTPA, exhibiting an affinity constant of 3.34 + or - 0.24 x 10(9) M(-1). Cross reactivity (CR) with other metals were below 1%, except for Fe(III) with a CR less than 5%. This quantitative indirect ELISA for the lead ion was used to detect environmental lead content in local water sources; importantly, the results from the immunoassay were in excellent agreement with those from ICP-MS. Development of immunoassays for metal ions may thus facilitate the detection and regulation of environmental pollution.

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