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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 16, 2026

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

An oligonucleotide microarray to characterize multidrug resistant plasmids.

Rebecca L Lindsey¹, Jonathan G Frye, Paula J Fedorka-Cray

¹U.S. Department of Agriculture, Agricultural Research Service, Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Richard B. Russell Agricultural Research Center, 950 College Station Road, Athens, GA 30604-2720, United States.

Journal of Microbiological Methods

|February 9, 2010

Summary

This study developed a novel microarray to rapidly screen for multiple drug resistance (MDR) genes on Inc A/C and Inc H1 plasmids. This method aids in understanding MDR plasmid transmission and its impact on antimicrobial therapy outcomes.

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Last Updated: Jun 16, 2026

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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Enterobacteriaceae frequently harbor multiple drug resistance (MDR) genes on Inc A/C and Inc H1 plasmids.
Understanding the transmission of these MDR plasmids is crucial for effective antimicrobial therapy.
Plasmids carrying MDR genes significantly influence treatment outcomes.

Purpose of the Study:

To design and validate a microarray for comprehensive gene content analysis of Inc A/C and Inc H1 plasmids.
To create a cost-effective and rapid screening method for MDR plasmid-associated genes.
To investigate the dynamic changes in plasmid gene content during transmission.

Main Methods:

Designed a microarray with 493 unique oligonucleotide probes (70 nucleotides in length) targeting all genes in six Inc A/C and one Inc H1 plasmid types.
Represented all redundant sequences only once to ensure specificity.
Hybridized control strains (Salmonella enterica, Escherichia coli) and test plasmids to the microarray.

Main Results:

The developed plasmid microarray enables high-density screening of bacterial isolates.
Demonstrated a rapid and cost-effective method for evaluating the gene content of Inc A/C and H1 plasmids.
Provided insights into how plasmid content evolves with transmission.

Conclusions:

The designed microarray is a valuable tool for studying MDR plasmid epidemiology.
Facilitates rapid identification of resistance genes and tracking of plasmid dissemination.
Supports better understanding of antimicrobial resistance mechanisms and transmission dynamics.