Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Sputum Studies II: Culture and Sensitivity

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...

Suctioning the Oropharyngeal Airway

Suctioning the Oropharyngeal Airway

In preparing for oropharyngeal airway suctioning, a nurse must gather all necessary equipment, including a suction unit with tubing, a prepackaged suction kit, sterile gloves, water or saline for irrigation, a water-soluble lubricant, and additional personal protective equipment (such as a gown, mask, and goggles) to control infections.
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...

Tracheostomy Suctioning II: Procedure

Tracheostomy Suctioning II: Procedure

Tracheostomy suctioning is a vital nursing procedure that involves removing secretions from the tracheostomy tube to maintain airway patency and prevent respiratory complications. Nurses need to understand the proper technique for tracheostomy suctioning to ensure patient safety and comfort. In this guide, we will outline the step-by-step process for performing tracheostomy suctioning, including preparing the sterile field, donning personal protective equipment (PPE), lubricating and connecting...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reply to "Olfactory Function and Biologic Treatments: A Comment on Available Real-life Studies".

Journal of investigational allergology & clinical immunology·2023

Same author

The Relation of CFTR-Genotype and Associated Comorbidities to Development of Pulmonary Atelectasis in Cystic Fibrosis Patients.

Archivos de bronconeumologia·2022

Same author

Improvement in Olfaction in Patients With CRSwNP and Severe Asthma Taking Anti-IgE and Anti-IL-5 Biologics: A Real-Life Study.

Journal of investigational allergology & clinical immunology·2022

Same author

Inconclusive diagnosis after a positive newborn bloodspot screening result for cystic fibrosis; clarification of the harmonised international definition.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2019

Same author

Cardiopulmonary fitness is strongly associated with body cell mass and fat-free mass: The Study of Health in Pomerania (SHIP).

Scandinavian journal of medicine & science in sports·2018

Same author

Host-pathogen diversity in a wild system: Chondrilla juncea-Puccinia chondrillina.

Oecologia·2017

Same journal

[Annual report of Anales de Pediatría editors].

Anales de pediatria (Barcelona, Spain : 2003)·2024

Same journal

[COVID-19: Critical appraisal of the evidence].

Anales de pediatria (Barcelona, Spain : 2003)·2024

Same journal

[Usefulness of chest ultrasound in a neonatal infection due to SARS-CoV-2].

Anales de pediatria (Barcelona, Spain : 2003)·2024

Same journal

[Cutaneous manifestations in the current pandemic of coronavirus infection disease (COVID 2019)].

Anales de pediatria (Barcelona, Spain : 2003)·2024

Same journal

[Breastfeeding app updates and recommendations].

Anales de pediatria (Barcelona, Spain : 2003)·2024

Same journal

[The impact of the pandemic on vaccination coverage in Spain: a challenge for pediatrics and public health].

Anales de pediatria (Barcelona, Spain : 2003)·2024

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Methodology for Sputum Induction and Laboratory Processing

Methodology for Sputum Induction and Laboratory Processing

Published on: December 17, 2017

[Sputum induction in children: Technical development].

G Vizmanos Lamotte¹, A Moreno Galdó, M J Cruz Carmona

¹Hospital Nostra Senyora de Meritxell, Escaldes-Engordany, Andorra. 32094gvl@comb.es

Anales De Pediatria (Barcelona, Spain : 2003)

|February 9, 2010

Summary

This summary is machine-generated.

High-flow nebulizers improve sputum induction success in children with asthma compared to low-flow devices. This method yields better sample quality with fewer side effects like cough and itchy eyes, making it a more effective technique.

Related Experiment Videos

Last Updated: Jun 16, 2026

Methodology for Sputum Induction and Laboratory Processing

Methodology for Sputum Induction and Laboratory Processing

Published on: December 17, 2017

Area of Science:

Pediatric Pulmonology
Respiratory Medicine
Medical Device Technology

Background:

Sputum induction is crucial for diagnosing respiratory conditions in children.
Optimizing sputum induction techniques can improve diagnostic yield and patient comfort.

Purpose of the Study:

To compare the performance and side effects of low-flow versus high-flow ultrasonic nebulizers for sputum induction in pediatric asthma patients.

Main Methods:

Sputum induction utilized hypertonic saline (3-5%) with either low-flow (1 ml/min) or high-flow (2.5-3 ml/min) ultrasonic nebulizers.
49 asthmatic children (7-15 years) underwent sputum induction procedures.

Main Results:

High-flow nebulizers achieved significantly higher test performance (85.3% vs. 53%, p=0.04) and valid sample rates (69% vs. 62.5%).
Incidence of cough and itchy eyes decreased with high-flow nebulizers, though bad taste and salivation increased.
Despite some increased discomforts, overall test performance was superior with high-flow devices.

Conclusions:

High-flow ultrasonic nebulizers enhance sputum induction performance in asthmatic children.
The technique offers improved sample quality without a significant increase in adverse events, making it a preferred method.