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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Published on: October 9, 2014

SOX2 modulates alternative splicing in transitional cell carcinoma.

Chun-Liang Tung1, Pei-Hsuan Hou, Yung-Ling Kao

  • 1Department of Pathology, Chia-Yi Christian Hospital, Chiayi, Taiwan.

Biochemical and Biophysical Research Communications
|February 9, 2010
PubMed
Summary

Aberrant alternative splicing is implicated in cancer. This study reveals sex-determining region Y-box protein 2 (SOX2) acts as a splicing factor, modulating alternative splicing in transitional cell carcinoma (TCC).

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant alternative splicing of cellular regulators is linked to cancer development.
  • Transitional cell carcinoma (TCC) is a significant form of cancer where splicing alterations may play a role.

Purpose of the Study:

  • To investigate the influence of altered alternative splicing on TCC development.
  • To identify specific splicing alterations and regulatory factors in TCC.

Main Methods:

  • Splicing activity was examined in TCC cell lines (TSGH8301, BFTC905) and a reference uroepithelial cell line (SV-HUC-1).
  • Gene expression profiling and validation were used to identify upregulated genes.
  • In vivo splicing reporter assays and in vitro pull-down assays were performed.

Main Results:

  • Significant alterations in splice site selection were observed in TCC cell lines.
  • Sex-determining region Y-box protein 2 (SOX2) was found to be specifically upregulated in the BFTC905 TCC cell line.
  • Ectopic SOX2 expression modulated alternative splicing, and SOX2 demonstrated RNA-binding capability, suggesting it functions as a splicing factor.

Conclusions:

  • SOX2 plays a role in modulating alternative splicing in transitional cell carcinoma.
  • SOX2's function as a splicing factor may contribute to TCC development.
  • Targeting SOX2-mediated alternative splicing could be a potential therapeutic strategy for TCC.