Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2-Methoxyestradiol Suppresses Stemness and Epithelial-Mesenchymal Transition in Drug-Resistant Esophageal Squamous Cell Carcinoma by Targeting the Snail Signaling Pathway in Cancer Stem Cells.

Stem cell reviews and reports·2026
Same author

Ion-shielding ultrathin encapsulation with hot-press bonded interface enables chronic stretchable bioelectronics.

Science advances·2026
Same author

Nanoplex cluster-mediated synergistic modulation of dendritic cells and T cells amplifies tumor-specific adaptive immunity.

Biomaterials·2026
Same author

Microchannel-containing hydrogel scaffolds enabled functional recovery in the absence of cells and bioactive molecules following spinal cord injury.

Journal of materials chemistry. B·2025
Same author

Customizable Fabrication of 2D and Conformal Multielectrode Arrays for 3D Printed Organotypic Bioelectronic Interfaces.

Advanced healthcare materials·2025
Same author

Sustained MicroRNA delivery enhanced remyelination and functional recovery after spinal cord injury.

Biomaterials·2025

Related Experiment Video

Updated: Jun 16, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

RNA interference by nanofiber-based siRNA delivery system.

Haoqing Cao1, Xu Jiang, Chou Chai

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 637459, Singapore.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|February 9, 2010
PubMed
Summary

This study introduces a novel scaffold-mediated approach for delivering small interfering RNA (siRNA) using polycaprolactone nanofibers. This method enables sustained, long-term gene silencing for applications in tissue engineering and regenerative medicine.

More Related Videos

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
05:36

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Published on: October 4, 2024

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Related Experiment Videos

Last Updated: Jun 16, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
05:36

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Published on: October 4, 2024

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Regenerative Medicine

Background:

  • Small interfering RNA (siRNA) is crucial for treating genetic diseases but requires effective delivery systems.
  • Current nanoparticle-based siRNA delivery faces limitations for long-term applications.
  • Tissue engineering and regenerative medicine necessitate advanced methods for sustained therapeutic molecule delivery.

Purpose of the Study:

  • To develop and evaluate a scaffold-mediated approach for long-term siRNA delivery.
  • To assess the bioactivity and gene-silencing efficiency of siRNA released from polycaprolactone (PCL) nanofibers.
  • To explore the potential of nanofibrous scaffolds for enhanced cellular uptake and gene silencing in regenerative medicine.

Main Methods:

  • siRNA was encapsulated within polycaprolactone (PCL) nanofibers (300-400nm diameter).
  • Controlled release of intact siRNA was measured under physiological conditions for up to 28 days.
  • HEK 293 cells were transfected with siRNA released from scaffolds, and gene-silencing efficiency was quantified.
  • Cellular uptake and gene silencing were assessed using direct cell seeding on biofunctional scaffolds.

Main Results:

  • Sustained release of intact siRNA from PCL nanofibers was achieved for at least 28 days.
  • Released siRNA remained bioactive, demonstrating 61-81% GAPDH gene-silencing efficiency comparable to conventional methods.
  • Direct cell seeding on scaffolds enhanced cellular uptake and facilitated efficient gene silencing.

Conclusions:

  • Nanofibrous scaffolds offer a promising platform for scaffold-mediated, long-term siRNA delivery.
  • This approach maintains siRNA bioactivity and achieves efficient gene silencing for regenerative medicine.
  • The combination of topographical and biochemical cues from scaffolds supports cellular development and therapeutic applications.