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Updated: Jun 16, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Characterization of a mannose utilization system in Bacillus subtilis.

Tianqi Sun¹, Josef Altenbuchner

¹Institut für Industrielle Genetik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.

Journal of Bacteriology

|February 9, 2010

Summary

Mannose metabolism in Bacillus subtilis is regulated by ManR, a transcription activator. Mannose induces mannose operon and manR transcription, but is also subject to carbon catabolite repression.

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Area of Science:

Microbiology
Molecular Biology
Gene Regulation

Background:

The mannose operon in Bacillus subtilis facilitates mannose transport and utilization.
A regulatory gene, manR, encodes a transcription activator for the mannose operon.

Purpose of the Study:

To elucidate the regulatory mechanism of the mannose operon in Bacillus subtilis.
To identify the role of ManR in transcriptional activation and regulation.

Main Methods:

Analysis of gene expression using lacZ reporter fusions.
Deletion analysis to identify DNA binding sites.
Protein sequence alignment to identify functional domains.
Mutational analysis to investigate regulatory pathways.

Main Results:

Mannose induces both mannose operon and manR transcription.
ManR binds to target sequences upstream of both promoters.
The phosphotransferase system (PTS) component EII(Man) negatively regulates the operon.
Carbon catabolite repression (CCR) affects both promoters.
ManR possesses PTS regulation domains (PRDs) and interacts with HPr for activation.
CCR involves HPr phosphorylation and CcpA complex formation.

Conclusions:

ManR acts as a mannose-inducible transcription activator for the mannose operon.
Regulation involves interplay between PTS, CCR, and ManR.
Phosphorylation of HPr is crucial for ManR-mediated transcriptional activation.