Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Canonical and noncanonical heterotrimeric G-protein signaling in uveal melanoma: Established mechanisms and emerging spindle-orientation hypotheses.

Biochemical pharmacology·2026

Same author

STIMATE-Mustn1 Fusion Protein Prevents Ca<sup>2+</sup> Overload in Cells That Present Increased SOCE.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Orai channels in proliferation, invasion, and chemoresistance of tumor cells.

American journal of physiology. Cell physiology·2026

Same author

Notch1 regulates Orai1 and Orai3 expression in breast cancer cells.

Scientific reports·2025

Same author

Orai1 plays a critical role in Orai3 synthesis and stability in luminal breast cancer cells.

Cell calcium·2025

Same author

Filamin A C-terminal fragment modulates Orai1 expression by inhibition of protein degradation.

American journal of physiology. Cell physiology·2025

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

SERCA2b activity is regulated by cyclophilins in human platelets.

Juan A Rosado¹, Jose A Pariente, Gines M Salido

¹Department of Physiology, University of Extremadura, Avd. Universidad s/n, Cáceres 10071, Spain.

Arteriosclerosis, Thrombosis, and Vascular Biology

|February 9, 2010

Summary

Cyclophilin A regulates sarcoendoplasmic calcium (Ca2+) adenosine triphosphatase (SERCA) activity in human platelets. Inhibition of cyclophilin A reduces SERCA2b calcium uptake, revealing a novel regulatory role.

More Related Videos

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

Related Experiment Videos

Last Updated: Jun 16, 2026

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

Area of Science:

Biochemistry
Molecular Biology
Platelet Physiology

Background:

Cyclophilins are chaperone proteins widely expressed in various cell types, including human platelets.
Sarcoendoplasmic calcium adenosine triphosphatase (SERCA) pumps are crucial for regulating intracellular calcium levels.
The specific role of cyclophilins in SERCA activity within platelets remains largely unexplored.

Purpose of the Study:

To investigate the influence of cyclophilins on the activity of sarcoendoplasmic calcium adenosine triphosphatase (SERCA) in human platelets.
To determine if cyclophilin inhibition affects specific SERCA isoforms or other calcium transport proteins in platelets.

Main Methods:

Utilized cyclosporin A (CsA) and its analogues to inhibit cyclophilins.
Assessed calcium (Ca2+) uptake by SERCA2b and other Ca2+-ATPases in platelet preparations.
Employed coimmunoprecipitation assays to identify protein interactions between cyclophilin A, SERCA2b, and stromal interaction molecule-1.

Main Results:

Cyclophilin inhibition with CsA dose-dependently reduced Ca2+ uptake by SERCA2b in human platelets.
SERCA3 and plasma membrane Ca2+-ATPase activity were unaffected by CsA treatment.
Coimmunoprecipitation confirmed cyclophilin A associates with SERCA2b in resting platelets, with altered interaction upon agonist stimulation or drug treatment.

Conclusions:

Cyclophilin A acts as a direct regulator of SERCA2b activity in human platelets.
This interaction highlights a novel mechanism controlling calcium homeostasis in platelets.
Findings suggest potential therapeutic targets for platelet-related disorders.