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Relative workload determines exercise-induced increases in PGC-1alpha mRNA.

Nikolai Baastrup Nordsborg1, Carsten Lundby, Lotte Leick

  • 1Copenhagen Muscle Research Centre, University of Copenhagen, Denmark. nnordsborg@ifi.ku.dk

Medicine and Science in Sports and Exercise
|February 9, 2010
PubMed
Summary
This summary is machine-generated.

High-intensity intermittent exercise significantly boosts metabolic mRNA, like peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha), in skeletal muscle. The relative workload, not absolute, is key for these exercise-induced gene expression changes.

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Area of Science:

  • Exercise Physiology
  • Molecular Biology
  • Skeletal Muscle Metabolism

Background:

  • Investigated the relationship between exercise intensity and metabolic mRNA expression in human skeletal muscle.
  • Examined the hypothesis that exercise-induced mRNA changes depend on relative workload.

Purpose of the Study:

  • To determine if relative workload influences the increase in skeletal muscle metabolic mRNA after brief intermittent exercise.
  • To compare the effects of different exercise intensities on gene expression in trained and untrained individuals.

Main Methods:

  • Trained and untrained subjects performed intermittent cycling exercise at 85% of VO2peak.
  • Trained subjects also exercised at an absolute workload equivalent to 70% of VO2peak.

Main Results:

  • Exercise at 85% VO2peak significantly increased plasma lactate and PGC-1alpha mRNA in both groups.
  • A lower intensity (70% VO2peak) resulted in less elevation of lactate and PGC-1alpha mRNA.
  • Pyruvate dehydrogenase kinase 4 and hexokinase II mRNA increased only at 85% VO2peak.

Conclusions:

  • Relative exercise intensity is crucial for exercise-induced increases in specific metabolic mRNAs, including PGC-1alpha.
  • Brief intermittent exercise at higher relative intensities drives greater changes in skeletal muscle gene expression.