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Cytotoxicity and genotoxicity reporter systems based on the use of mammalian cells

Christa Baumstark-Khan1, Christine E Hellweg, Günther Reitz

  • 1Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Centre (DLR), Linder Hoehe, 51147, Cologne, Germany, christa.baumstark-khan@dlr.de.

Advances in Biochemical Engineering/Biotechnology
|February 9, 2010
PubMed

Insights

New reporter assays are crucial for quickly assessing risks from industrial chemicals. This review covers cytotoxicity, genotoxicity testing, and cellular reporter assay design for toxicology screening.

Area of Science:

  • Toxicology and Environmental Health
  • Biotechnology and Molecular Assays

Background:

  • Increasing industrial agents necessitate rapid risk assessment methods.
  • Conventional cytotoxicity and genotoxicity tests require enhancement for modern needs.

Purpose of the Study:

  • To review strategies for high-content screening in toxicology.
  • To discuss the design and application of novel reporter assays.

Main Methods:

  • Review of conventional and advanced toxicology screening methods.
  • Examination of genetic strategies for designing cellular reporter assays.
  • Presentation of current luminescent/fluorescent reporter assay designs.

Main Results:

  • Cellular reporters utilize stress-regulated gene promoters linked to reporter genes.
  • Reporter assays offer a sensitive method for detecting chemical toxicity.
  • Genetic engineering enables tailored assay development for specific risks.

Conclusions:

  • Reporter assays are vital for efficient toxicological evaluation.
  • Advanced screening strategies improve risk assessment for man and environment.
  • Cellular reporter systems represent a significant advancement in toxicology.

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