Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

ABX464 (obefazimod) for patients with COVID-19 at risk for severe disease: miR-AGE, a randomized, double-blind placebo-controlled trial.

The journal of allergy and clinical immunology. Global·2023

Same author

Demonstration of the Antitumor Activity of the iNKT Agonist ABX196, a Novel Enhancer of Cancer Immunotherapy, in Melanoma and Hepatocarcinoma Mouse Models.

Molecular cancer therapeutics·2022

Same author

ABX464 (obefazimod) for moderate-to-severe, active ulcerative colitis: a phase 2b, double-blind, randomised, placebo-controlled induction trial and 48 week, open-label extension.

The lancet. Gastroenterology & hepatology·2022

Same author

Safety, tolerability and impact on viral reservoirs of the addition to antiretroviral therapy of ABX464, an investigational antiviral drug, in individuals living with HIV-1: a Phase IIa randomised controlled study.

Journal of virus eradication·2019

Same author

Vero cell technology for rapid development of inactivated whole virus vaccines for emerging viral diseases.

Expert review of vaccines·2017

Same author

The Anti-Hiv Candidate Abx464 Dampens Intestinal Inflammation by Triggering Il-22 Production in Activated Macrophages.

Scientific reports·2017

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Published on: November 21, 2023

Developing cell culture-derived pandemic vaccines.

P Noel Barrett¹, Daniel Portsmouth, Hartmut J Ehrlich

¹Baxter BioScience, Global R&D, Biomedical Research Centre, Orth/Donau, Austria. noel_barrett@baxter.com

Current Opinion in Molecular Therapeutics

|February 9, 2010

Summary

Traditional egg-based vaccine production may not meet global demand during pandemics. Cell culture-derived influenza vaccines offer faster, more efficient production and improved immunogenic efficacy, addressing these limitations.

More Related Videos

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

Related Experiment Videos

Last Updated: Jun 16, 2026

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Published on: November 21, 2023

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

Area of Science:

Virology
Vaccinology
Biotechnology

Background:

Emerging influenza strains (avian, swine-origin A/H1N1) raise concerns about pandemic preparedness.
Existing egg-based influenza vaccine manufacturing may be insufficient for global demand during a pandemic.

Purpose of the Study:

To review modern cell culture-derived influenza vaccines and their manufacturing processes.
To highlight the advantages of cell culture vaccines over traditional methods.

Main Methods:

Detailed description of vaccine production technologies: mammalian cell lines (Vero, MDCK, PER.C6) and baculovirus/insect cell platform.
Review of clinical data for advanced-stage cell culture-derived vaccines.
Inclusion of preclinical evaluation of experimental technologies.

Main Results:

Cell culture-derived influenza vaccines demonstrate advantages in production speed and efficiency.
These vaccines show promising immunogenic efficacy.
Multiple cell line and platform technologies are advancing.

Conclusions:

Cell culture-derived influenza vaccines represent a viable and potentially superior alternative to egg-based production.
These modern platforms enhance global capacity for rapid vaccine response to influenza pandemics.