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Updated: Jun 16, 2026

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
Chicken meat is an infection source of Salmonella serovar Infantis for humans in Japan
Tamie Noda1, Koichi Murakami, Yasuhisa Ishiguro
1Division of Pathology and Bacteriology, Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan. noda-t8031@pref.fukuoka.lg.jp
Background:
The study sets out to either confirm or refute a recent study's findings that chicken meat is an unlikely source of Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) in humans in the Kyushu-Okinawa region, Japan.
Methods:
A total of 74 Salmonella Infantis isolates (30 from human and 44 from other sources), mainly from the Kyushu-Okinawa region in south-western Japan, were analyzed using a molecular-epidemiological approach combining two fingerprinting methods, namely pulsed-field gel electrophoresis and amplified fragment length polymorphism (AFLP), a novel polymerase chain reaction-based technique.
Results:
The resulting pulsed-field profiles showed that 17 of 30 human isolates were similar to those found in chicken meat, whereas there were no common pulsed-field profiles between human and chicken egg isolates. Overall, 3 of 18 AFLP profiles included 7 human isolates and 14 chicken egg isolates. In addition, the combined results of the pulsed-field gel electrophoresis and AFLP analyses showed that 8 human Salmonella Infantis and 13 chicken meat isolates belonged to the same clonal lines.
Conclusions:
These results suggest that chicken meat is an infection source of Salmonella Infantis for humans in the Kyushu-Okinawa region, Japan. The results also showed the relatively high suitability of AFLP for application to epidemiological studies of Salmonella Infantis.
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