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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Published on: October 16, 2016

Differential regional expression of genes in the developing brain of Ciona intestinalis embryos

Naoki Shimozono1, Naoyuki Ohta, Nori Satoh

  • 1Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan. shimozono.n@at8.ecs.kyoto-u.ac.jp

Zoological Science
|February 10, 2010
PubMed

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Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...