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Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic
J Welsh1, C Petersen, M McClelland
1California Institute of Biological Research, La Jolla 92037.
Nucleic Acids Research
|January 25, 1991
Summary
Arbitrarily primed PCR (AP-PCR) genomic fingerprinting can identify mouse strains. This technique allows rapid genetic mapping of DNA polymorphisms, proving valuable for strain verification and genetic studies.
Area of Science:
- Genomics
- Molecular Biology
- Animal Genetics
Background:
- Genomic fingerprinting techniques like arbitrarily primed PCR (AP-PCR) offer a method for differentiating between organisms.
- Mouse (Mus musculus) strain identification and verification are crucial for research consistency.
Purpose of the Study:
- To apply AP-PCR for generating genomic fingerprints in mice.
- To evaluate the utility of AP-PCR for mouse strain identification and verification.
- To demonstrate the application of AP-PCR for genetic mapping of DNA polymorphisms.
Main Methods:
- Arbitrarily primed PCR (AP-PCR) was used to generate genomic fingerprints from mouse strains.
- One primer was utilized to amplify DNA fragments, creating characteristic banding patterns.
- Genetic mapping of polymorphisms was performed using C57BL/6J x DBA/2J recombinant inbred mice.
Main Results:
- AP-PCR generated distinct genomic fingerprints capable of differentiating between mouse strains.
- Four polymorphisms were genetically mapped using a single primer.
- One identified polymorphism was a DNA length variant.
Conclusions:
- AP-PCR is a valuable tool for mouse strain identification and verification.
- The method enables rapid genetic mapping of DNA polymorphisms without the need for Southern blotting.
- This technique facilitates efficient genetic analysis in mouse models.