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Updated: Jun 16, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
Optimized synthesis of phosphatidylserine
Giuseppe Guanti1, L Banfi, A Basso
1Dipartimento di Chimica e Chimica Industriale, Università di Genova, via Dodecaneso 31, 16146, Genoa, Italy. guanti@chimica.unige.it
Abstract:
The synthesis of phosphatidyl serine containing saturated fatty acids was thoroughly studied and optimized in order to establish a protocol amenable to large-scale synthesis. The key step was a one-pot multicomponent reaction involving an O-benzyl phosphorodiamidite, protected serine and diacylglycerol, followed by in situ oxidation of the resulting phosphite. In order to replace expensive and poorly stable tetrazole, a screening of substitutes was carried out and imidazolium chloride was selected as the best suited one.
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